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BigHeart maps an adult human heart in 3D at 8.01-micron resolution

A bioRxiv preprint presents a continuous three-dimensional structural map of a whole adult human heart at 8.01-micron isotropic voxel size. The dataset brings myocardial structure, electrical conduction tissue, blood vessels, lymphatic collectors and autonomic nerves into one spatial framework.

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Preprint reports workflow to reduce autofluorescence in aged human brain tissue

A bioRxiv preprint reports a Tris-EDTA-supplemented photobleaching workflow that reduced autofluorescence by 58–70% in fresh-frozen aged human brain sections while preserving tissue architecture and cellular content. The study also introduces a 28-plex antibody panel for spatial proteomics.

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Bio-CM2 Combines Fluorescence and Reflectance in a Miniature Mesoscope

A bioRxiv preprint describes Bio-CM2, a miniature mesoscope combining fluorescence and label-free reflectance imaging across a 7.5 × 10 mm² field of view. Demonstrations in worms, zebrafish and mice paired molecular signals with morphology, behaviour and physiology.